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Atmospheric Pressure Mass Spectrometry

SunCollect MALDI Spotter and Tissue prepration device

The SunCollect MALDI spotter from SunChrom (Friedrichsdorf, Germany) is a flexible and extremely accurate fraction-collector and MALDI target-spotter. Fractions can be collected from either micro or nano HPLC runs and spotted onto the most common MALDI target plates or microtitre plate wells. Superb precision ensures that there is no contamination between neighbouring spots and all materials are biocompatible. With the use of its precise spraying accessory, the SunCollect is ideal for preparing tissue sections for MALDI imaging

SunCollect MALDI Spotter

Spot or collect LC fractions, or prepare tissues for imaging

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Features

  • Simultaneous collection of a sample on a microtiter plate and a MALDI Target
  • Capability for on-tissue digests making it ideal for MALDI imaging applications - no probe blockages
  • Probe moves from one spot to another in less than 1 second
  • Compact unit 36 x 30 x 26 cm (w,d,h)
  • Holds multiple MALDI target plates or microscope slides
  • Biocompatible - sample is only in contact with PEEK or quartz components

See a short presentation on the SunCollect features, SunCollect Features.

On-tissue petide digests

FFPE breast tumour tissue section. MALDI MS Images of the distribution of in situ digested peptides within the section

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Tissue Preparation for Imaging MALDI

Tissue samples can be easily prepared for MALDI imaging using the SunCollect. The spotter can handle fresh-frozen or FFPE tissue sections. Enzymes such as trypsin can be applied to the tissue for in-situ digestion with a matrix solution. Shown below are images obtained from FFPE breast cancer xenograft tissue prepared by Marie-Claude Djidja in Professor Malcolm Clench's laboratory at the Sheffield Hallam University. These were then run on a Waters Synapt HDMS mass spectrometer at the Waters MicroMass applications laboratory in Manchester

Click here to see an ASMS poster presention on Tissue imaging.

 
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KR Analytical Ltd
77 Forge Fields
Sandbach
Cheshire
CW11 3RN
UK