The SunCollect MALDI spotter from SunChrom (Friedrichsdorf, Germany) is a flexible and extremely accurate fraction-collector and MALDI target-spotter. Fractions can be collected from either micro or nano HPLC runs and spotted onto the most common MALDI target plates or microtitre plate wells. Superb precision ensures that there is no contamination between neighbouring spots and all materials are biocompatible. With the use of its precise spraying accessory, the SunCollect is ideal for preparing tissue sections for MALDI imaging
See a short presentation on the SunCollect features, SunCollect Features.
Tissue samples can be easily prepared for MALDI imaging using the SunCollect. The spotter can handle fresh-frozen or FFPE tissue sections. Enzymes such as trypsin can be applied to the tissue for in-situ digestion with a matrix solution. Shown below are images obtained from FFPE breast cancer xenograft tissue prepared by Marie-Claude Djidja in Professor Malcolm Clench's laboratory at the Sheffield Hallam University. These were then run on a Waters Synapt HDMS mass spectrometer at the Waters MicroMass applications laboratory in Manchester
Click here to see an ASMS poster presention on Tissue imaging.
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