MALDI Atmospheric Pressure Mass Spectrometry
Sample preparation for MALDI
Sample preparation for MALDI
Tissue samples can be easily prepared for MALDI imaging using the SunCollect. The sprayer can handle fresh-frozen or FFPE tissue sections. Enzymes such as trypsin and other derivatisation liquids can be applied to the tissue for in situ digestion.
The SunDigest enables precise and accurate control of temperature and humidity to optimise the tissue digestion/modification.
Features
- Automated delivery of matrix, enzyme or wash solutions
- 6 port PEEK valve with low dead-volume to minimise liquid usage
- Self cleaning at end of matrix application
- Compact unit 36 x 30 x 26 cm (w,d,h)
- 5 sample slides can be sprayed simultaneously
- Easy to use software
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Applications and Data Examples
Maldi Imaging
Add AP-MALDI to your LC-MS instrument and preform Mass Spec Imaging (MSI) at a fraction of the cost of a dedicated MALDI Mass Spectrometer. The MassTech AP-MALDI source can be added to ThermoFisher Scientific, Agilent, AB-Sciex LC-MS instruments to give you MALDI imaging capability.
Tissue Imaging
The data to the right was produced using an AP‑MALDI UHR / Orbitrap Exploris 480 HRMS imaging experiment performed by Dr. Gilles Frache, Luxembourg Institute of Science and Technology (LIST) and Maureen Feucherolles ( LIST). The mouse brain was obtained through the courtesy of Pr. William J Griffiths Pr. Yuqin Wang (Swansea University Oxysterol Research Group) and the cryo-sections have been prepared for analysis using the SunChrom matrix sprayer. The tissue was imaged at 25 micrometer pixel size at a mass resolution of 480 000 at m/z 200.
The image shows the RGB overlay image of 3 lipids. Red: [C40H79O10P + Na]⁺ m/z=773.5303 tentatively assigned as PG(34:0), Green: [C41H82NO8P + Na]⁺ m/z=770.5670 tentatively assigned as PE(36:0), Blue: [C46H89NO8 + H]⁺ m/z=784.6661 tentatively assigned as GalCer(40:1) according to Metaspace http://www.nature.com/nmeth/journal/v14/n1/full/nmeth.4072.html
The data set is available on https://metaspace2020.eu/datasets?prj=f62682cc-5ebd-11ee-adaa-33f255ad8aae
One of the uses of MALDI tissue imaging in the pharmaceutical industry is to monitor the distribution of drugs and their metabolites in target tissues. This interesting application note shows the use of the AP-MALDI source with a high resolution mass spectrometer analysing tissues that have been prepared for analysis using the SunCollect spraying device.
Documentation
Download: App-Note KRA-peptides
Download: APMALDI mouse brain imaging on Exploris480
Download: APMALDI on Exploris480